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Abstract

The aim of study is to verify biofilm forming capacity of P. aeruginosa isolates from different clinical sources and its association with antibiotics resistance. In the current study, fifty (50) P. aeruginosa isolates were recovered from 250 clinical specimens collected from three main hospitals in Diyala Province. The results revealed that 64% of isolates were MDR and 18% XDR towards many antibiotics used 7 antibiotics including Piperacillin-tazobactam, Ceftazidim, Cefepem, Azetronam, Meropenem, Amikacin and Ciprofloxacin. The rates of resistance toward these antibiotics were 66%, 80%, 88%, 72%, 54%, 52% and 40% respectively. The (MIC) for Polymyxin B ranged from 2- 16 mg/ml. Biofilm-production was assessed the Micro-titer plate quantitative method showed that all of isolates were biofilm producers. There were differences in the biofilm-production distributed into 16 (32%) were strong biofilm producer 19 (38%) moderate, and 15 (30%) were weak. The capability of P. aeruginosa to form biofilms on expired human blood and serum was also detected, and the results showed that all isolates were 100% biofilm producers. The separated red blood corpuscles (RBCs) had a higher rate of biofilm formation than serum, and the results also varied between blood groups where blood group AB was the highest in productivity, followed by O, B and A group respectively. Biofilm formation results on blood serum revealed that blood group O and AB, were the highest biofilm forming among the blood groups, followed by serum A and B, respectively.

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