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Abstract

The aim of this study is to assess the biofilm-production rates in clinical S. aureus isolates and to correlate between multidrug resistance and their biofilm-forming capacity. One hundred ninety (190) clinical specimens were collected from different clinical sources (wounds, burns, urine, ear and blood) in Diyala province, during the period from September 15/ 2022 to December 15/ 2022. The isolates were diagnosed morphologically, microscopically, biochemically and confirmed by 16SrRNA detection. All isolates were investigated for antibiotic sensitivity, using the disc diffusion method. The isolates under test showed a high resistance 100% to OX, while the rates of resistance ranged from C, F, AZM, VA, DA, TME, CN, RA, DOX, FEP, TEC 16%, 4%, 92%, 4%, 60%, 72%, 44%, 48%, 44%, 80%, and 42%, respectively. The ability of S. aureus isolates biofilm was investigated. All the isolates under study were coagulase producers and showed β-hemolysis. The results showed that all isolates were biofilm producer using micro-titer plate method.

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