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Abstract

Klebsiella pneumonia is a very deleterious microbes that is essentially take gram dye staining & considered gram negative when stained by gram staining method and appear microscopically as bar or rod in shape which have very mucoid large specific colonies on blood agar medium due to potential ability to produce large glycocalyx and muco- polysaccharides that link together to secrete large biofilm and slime layer which in turn increase invasive ability of microbes to host and become resistant to several effective drugs , increase these ability being widely observed in highly evolutionary strains that have multi-virulent factors like muco-polysaccharide capsule , slime layer , biofilm in association with extremely drug resistant genes , so these bacterial strains now is demonstrated as hypervirulent Klebsiella pneumonia (hvKp) involved in several chronic infections worldwide .To identify the new resistant hypervirulent strains of Klebsiella pneumonia in hospitalized patients suffering from chronic urinary tract infections (UTI) along with catheters related patients and to determine the ability of these dangerous strains to resist strong effective antibiotics like Imipenem .Urine samples were collected from 150 urinary tract infection patients admitted in different hospitals and specialized centers of Baghdad province , then all specimens were manipulated in laboratory and cultured in special bacterial media to improve Klebsiella pneumonia species and to exclude other bacteria , also further identification were done by selective tests like biochemical estimation of IMVIC and vitic-2 system along with biofilm determination and antibiotic sensitivity for certain antibacterial agents. This study have results that depend on hypervirulent ability of new emergence bacterial strains to resists the lethal action of strong bactericidal agents like imipenem, so 70% females and 30% males from 100 Klebsiella pneumonia isolates were 41% resistant to imipenem were as only 4% Esherishia.coli (E. coli) resistant strains to imipenem from a total of 150 urine samples (100 were Klebsiella pneumonia & 50 were E. coli). High percentage of imipenem resistance by Klebsiella pneumonia bacteria indicates that these isolates differ in virulence ability to classical nosocomial strains of klebsiella and have multi-resistant genes on their plasmid and chromosomal DNA that play essential role in wide distribution among peoples and from a medical points that become a very sever to be treated and controlled.

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