Abstract
Animal models are standard tools for studying a wide range of traumatic wound infections. This paper aims to study the effect of Staphylococcus aureus (SA) in the wound and how the innate immune system interacts with this infection, by measuring C5a, and there are roles on wound healing. Fiftyone (females) Albino Rats at the age 6-9 week. The animals were divided into three groups (17 animal in the group) Group I (healthy control), Group II (control wound), and Group III (infection contamination, wound group), To mimic clinically occurring infections caused by SA infection, excision wound was made on the dorsal side of the animals, and SA 2 × 108 (CFU)/mL was inoculated on the wound site in infection contamination group, The blood was collected after 24hours postinfection for three days (24, 48, 72 hours) post-infection, ELISA technique was used for Measure C5a levels . Immunological assay results of this study showed that the serum level for C5a was statistical significant between different values at different times with (P ≤0.04). The study successfully established the role of C5a in wound infection with SA.
First Page
131
Last Page
138
Recommended Citation
Al-khafaji, Athraa Hassan and Al-Shabaa, Kifah Fadhil
(2022)
"Measuring the levels of C5a in induced wound infection with Staphylococcus aureus,"
Al-Nisour Journal for Medical Sciences: Vol. 4:
Iss.
2, Article 13.
DOI: https://doi.org/10.70492/2664-0554.1083