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Abstract

Background: Pseudomonas aeruginosa, a human opportunistic Gram-negative pathogen, is one of the most important nosocomial pathogens and is a major health problem, primarily in immunocompromised individuals. Aims: To detect exoU and toxA genes in Pseudomonas aeruginosa isolates with PCR techniques and study their sequencing. Materials and Methods: 210 swabs were collected from patients suffering burns and wound infections in both genders, for the period from November 2019 to the end of February 2020. Results: After final diagnosis of samples, 50(23.80%) isolates of P. aeruginosa were obtained. The PCR was carried out for all bacterial isolates, using specialized primers, to detect these two genes (toxA and exoU). The results showed that the twenty P. aeruginosa isolates have the toxA gene in a percentage 40% from all isolates. The results also showed that the same 20 P. aeruginosa isolates have the gene exoU in a percentage 40% from all isolates. The toxA and exoU genes were amplified by PCR method; the sequencing result of exoU gene showd that P. aeruginosa having Two Transition G>A code GGC\AAC of amino acid Glycine > Asparagine and Predicted effect Missense, also one Transversion T >A code GAA>CAA amino acid change Leucine to Histidine the effect Missense. From the Gene Bank, found that part of exoU gene having 99% compatibility with subject of exoU gene. While the analysis of the exoU gene was coordinated by 99% having two Transition of G>A, and C>T code GGC>GAT and amino acid transformation Glycine\ Aspartic acid the effect Missense. The of sequencing for toxA gene shows Compatibility of 100% in Gene Bank of toxA gene, so no recorded change noticed. Conclusion: The P. aeruginosa which isolated from wounds and burns possessed virulence genes, toxA and exoU genes. The exoU and toxA genes having Transition, amino acid alteration and amino acid transformation.

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