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Abstract

A total of (100) patients attending the Center of Tuberculosis and Chest Disease in Baghdad were included in this study. Their ages ranged between (17-68) years. They all complained of a reproductive cough. The study was accomplished during the period extending from November 2019 till May 2020. Twenty-five were found to have M. tuberculosis by using the Ziehl Neelsen stain and culture technique (15 males, 10 females), their ages ranging between 17 and 68 years. Twenty-five age and sex-matched normal healthy-looking individuals were also chosen as control group for comparison. In this work, the concentrations of the cytokines, interleukine-12(IL-12) and tumor necrotic factor alpha (TNF-α) were evaluated in the sera of Tuberculoid patients by using Enzyme Linked Immunosorbent Assay (ELISA). Results of this work revealed that the mean concentrations of (IL-12 and TNF-α) were significantly higher in the sera of patients with M. tuberculosis infection when compared with their concentration in the sera of the control group (P<0.001). No significant differences were found in the mean concentration of serum (IL-12 and TNF-α) between males and females with M. tuberculosis infection (P>0.05).

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